EMBRYONIC CEREBROSPINAL FLUID (e-CSF), PROTEINS FROM e-CSF, AND RELATED METHODS AND COMPOSITIONS
AssigneeBeth Israel Deaconness Medical Center
InventorsChristopher A. Walsh, Mauro Zappaterra, Maria Lehtinen
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Abstract
We have performed a proteomic analysis of embryonic cerebrospinal fluid (e-CSF) in human and rats. Based on this discovery, the invention features methods and compositions for cell culture including components of e-CSF or fragments thereof. Also provided are methods for extraction of e-CSF.
Claims29
A composition comprising at least one component of e-CSF, wherein said component is present at an enhanced level relative to the level in e-CSF and said composition is capable of supporting proliferation, maintenance, or differentiation of a cultured cell.
A kit comprising:
- (a) a composition comprising at least one component of e-CSF, wherein the component is present at an enhanced level relative to naturally occurring e-CSF;
- and
- (b) instructions for using (a) for cell culture.
A method of culturing a stem cell or a progenitor cell, comprising incubating said cell in culture media containing at least one isolated component of rat or human e-CSF.
A method of isolating embryonic cerebrospinal fluid (e-CSF) comprising:
- (a) providing an embryo;
- (b) inserting a capillary needle into a ventricle of the central nervous system of said embryo such that the tip of said needle contacts CSF;
- and
- (c) extracting CSF from said embryo through said needle, thereby isolating e-CSF.
Dependencies are inferred from the claim text.