Laser scanning cytometry mediated analysis of therapeutic efficacy in tumors
AssigneeBoard of Regents, The University of Texas System
InventorsDARREN W. DAVIS, David J. McConkey
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Abstract
This invention describes the use of a laser scanning device, for example a laser scanning CYTOMETRY (LSC), with a double-fluorescent labeling technique as a quantitative method that can be used to objectively and accurately measure endothelial cell death, endothelial tumor cell death and blood vessel density of tumor tissue. These parameters can be used as markers of efficacy in tumors treated with anti-angiogenic or traditional therapies and can distinguish patients who respond to these drugs from those who do not.
Claims54
A method for predicting anti-cancer therapeutic efficacy in an animal with a cancerous tumor comprising:
- a) obtaining a cancer tumor tissue sample from an animal subject undergoing anti-cancer therapy;
- b) staining said sample with at least one fluorescent label, wherein the at least one fluorescent label stains nuclei;
- c) subjecting said sample to laser scanning cytometry;
- and
- d) obtaining data on the number of apoptotic endothelial cells, apoptotic tumor-associated endothelial cells, blood vessel density or cell proliferation in said sample,
- wherein predicting anti-cancer therapeutic efficacy is determined by comparing said data to similar data from said tissue sample obtained either at a different time or at a different location than first said tissue sample, wherein an increase in the number of apoptotic endothelial cells, an increase in the number of apoptotic tumor-associated endothelial cells, a decrease in the blood vessel density, and/or a decrease in cell proliferation predicts that said anti-cancer therapy will be therapeutically effective.
A kit comprising:
- a fluorescent label for endothelial cell markers, a fluorescent label for cell nuclei, TAQ polymerase and buffer for use in assessing anti-cancer therapeutic efficacy with laser scanning cytometry.
Dependencies are inferred from the claim text.