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US 2011/0236884 A1Published application
Microbubbles for affinity separation
OwnerEdward Jablonski, Thomas Adamsinventors
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Abstract
The present invention relates to methods, compositions and kits for affinity isolation, affinity purification and affinity assay based on microbubbles coated with an affinity molecule. Particularly, the invention provides protein microbubbles coated with an affinity molecule. In addition, the invention provides glass microbubbles coated with an affinity molecule. Methods of using the microbubbles of the invention for isolating analytes and cells are specifically provided.
Claims25
1-44. (canceled)
A method for affinity isolation or affinity assay of a species comprising:
- (a) providing microbubbles coated with an affinity molecule in a solution;
- (b) contacting the microbubbles with a species that interacts with the affinity molecule in a solution, thereby generating microbubbles coated with the species;
- and (c) allowing the microbubbles coated with the species to float to the top of the solution, thereby separating the microbubbles coated with the species from free species and the solution.
A method for depleting a solution of a species, comprising the steps of
- (a) providing, in a solution, albumin microbubbles coated with an affinity molecule that specifically binds the species;
- (b) contacting, in the solution, the microbubbles with the species that interacts with the
- affinity molecule coated on the microbubbles, thereby generating microbubbles coated with the species;
- (c) separating the microbubbles coated with the species from the solution, thereby depleting the solution of the species;
- and
- (d) detecting an analyte remaining in the solution.
3 more elements
Dependencies are inferred from the claim text.