Detection of herpes simplex virus types 1 and 2 by nucleic acid amplification
AssigneeBecton, Dickinson and Company
InventorsDavid M. Wolfe, Christine A. Martinaitis, Daretta A. Yursis
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Abstract
The present invention relates to a method of detecting the presence or absence of herpes simplex virus (HSV) in a sample based on amplifying a portion of the Glycoprotein G(US4) gene of HSV and detecting the presence of the amplified nucleic acid using primers and detector primers as described herewith. The method of the invention further identifies the type of HSV, either HSV-1 or HSV-2, in a sample. Also encompassed by the invention is a kit comprising the primers and detector primers which may be used with the amplification method described herewith.
Claims37
A polynucleotide comprising an HSV-2 target binding sequence consisting of the HSV-2 target binding sequence of any one of SEQ ID NOs:38, 43 and 45.
A kit for detecting an HSV-2 target sequence, comprising first, second and third primers having an HSV-2 target binding sequence consisting of the HSV-2 target binding sequence of SEQ ID NOs:38, 43 and 45 respectively.
A composition comprising primers for the detection of an HSV-2 target sequence in a sample by an amplification reaction comprising:
- (a) a first amplification primer which is SEQ ID NO:38;
- (b) a second amplification primer which is SEQ ID NO:43;
- (c) a first bumper primer that hybridizes to the HSV-2 target sequence upstream of the first amplification primer, wherein the first bumper primer is SEQ ID NO:46;
- (d) a second bumper primer that hybridizes to a complement of the HSV-2 target sequence upstream of the second amplification primer, wherein the second bumper primer is SEQ ID NO:47;
- and (e) a third primer having an HSV-2 target binding sequence consisting of the HSV-2 target binding sequence of SEQ ID NO:45.
A method of detecting the presence of HSV-2 target sequence in a sample, said method comprising:
- (a) adding the sample to a first amplification primer which is SEQ ID NO:38;
- a second amplification primer which is SEQ ID NO:43, and a third detectably labeled primer having an HSV-2 target binding sequence consisting of the HSV-2 target binding sequence of SEQ ID NO:45;
- producing an amplified HSV-2 nucleic acid product;
- and (b) detecting the amplified HSV-2 nucleic acid product, wherein the detection of the amplified product indicates the presence of HSV-2 in the sample.
A method of detecting an HSV-2 target sequence comprising:
- (a) amplifying the HSV-2 target sequence using a first amplification primer comprising an HSV-2 target binding sequence consisting of the HSV-2 target binding sequence of SEQ ID NO:38;
- (b) amplifying the target sequence using a second amplification primer comprising an HSV-2 target binding sequence consisting of the HSV-2 target binding sequence of SEQ ID NO:43;
- and (c) detecting the amplified target sequence with a third detectably labeled primer having an HSV-2 target binding sequence consisting of the HSV-2 target binding sequence of SEQ ID NO:45.
Dependencies are inferred from the claim text.