US 2011/0236882 A1 · QUANTITATIVE MEASUREMENT OF NANO/MICRO PARTICLE ENDOCYTOSIS WITH CELL MASS SPECTROMETRY

Abstract

Methods for detecting the presence of nanoparticles or microparticles by cell mass spectrometry (CMS) are provided. CMS methods are provided for determining the number of nanoparticles or microparticles in each cell. Nanoparticles whose intracellular concentration can be determines by the CMS methods of the invention include polymeric nanoparticles (NPs), liposomes, viral-based NPs, carbon nanotubes, diamond NPs, polymeric micelles, nanocarriers, liposomes, and viral nanoparticles. Determination of the efficiency of drug delivery and intracellular titer of pathogens according to the invention is disclosed. Methods for determining intracellular uptake of virus particles are provided.

Claims30

Dependent claims

A method for determining uptake of nanoparticles or microparticles by a cell, the method comprising the steps of:

(a) providing a cell contacted with a nanoparticle or microparticle of known dimension under conditions suitable for uptake of the nanoparticle or microparticle by the cell;
(b) recording a mass spectrum of the cell by cell mass spectrometry;
and
(c) determining a mass/charge (m/z) ratio of the cell.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

13/075,160Filed 29 Mar 2011

Priority

29 Mar 2010

Published

29 Sep 2011

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

G01N 33/5091
Involving human or animal cells: for testing the pathological state of an organism
G01N 33/5008
Involving human or animal cells: for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
G01N 33/569
Immunoassay; Biospecific binding assay; Materials therefor: for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/6848
General methods of protein analysis not limited to specific proteins or families of proteins: methods of protein analysis involving mass spectrometry

Quantitative measurement of nano/micro particle endocytosis with cell mass spectrometry

Life of this patent

Quantitative measurement of nano/micro particle endocytosis with cell mass spectrometry

Life of this patent

Abstract

Claims30