Mutant gluconate dehydrogenase

A mutant gluconate dehydrogenase made by an amino acid sequence obtained by deleting, substituting, adding or inserting one or plural amino acids in an amino acid sequence represented by sequence number 1,

• the mutant gluconate dehydrogenase displaying enzyme activity which is equal to or higher than 120% of that of a wild-type gluconate dehydrogenase made by an amino acid sequence represented by sequence number 1.

A mutant gluconate dehydrogenase made by an amino acid sequence obtained by deleting, substituting, adding or inserting one or plural amino acids in an amino acid sequence represented by sequence number 1,

• wherein residual enzyme activity after heat treatment performed at 47.5° C. for 10 minutes is equal to or higher than 20% of enzyme activity before the heat treatment.

An electrochemical device using enzyme, characterized in that the enzyme is a mutant gluconate dehydrogenase made by an amino acid sequence obtained by deleting, substituting, adding or inserting one or plural amino acids in an amino acid sequence represented by sequence number 1, and

• the mutant gluconate dehydrogenase displays enzyme activity which is equal to or higher than 120% of that of a wild-type gluconate dehydrogenase made by an amino acid sequence represented by sequence number 1.