Glyceroglycolipid lipase
AssigneeMitsubishi-Kagaju Foods Corporation
InventorsKaoru Tsukazaki, Tetsuya Fukazawa, Isshin Tanaka
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Abstract
Disclosed is a glyceroglycolipid lipase which is highly safe, can hydrolyze a neutral fat, a glycerophospholipid or a glyceroglycolipid at about pH 6, is thermally stable to some extent, can hydrolyze lecithin, cannot hydrolyze lysolecithin, can rise a bread when used singly in the production of the bread, and has no unpleasant odor. Specifically disclosed is a glyceroglycolipid lipase derived from a filamentous bacterium Aspergillus japonicus.
Claims19
A glyceroglycolipid lipase having the following properties:
- 1) having a molecular weight of approximately 29,000 as determined by SDS-PAGE;
- 2) hydrolyzing neutral fat, lecithin, and glyceroglycolipid at pH 6.0;
- 3) having a glyceroglycolipid degradation activity at least 10-fold higher than a lecithin degradation activity at pH ranging from 3.6 to 8.9.
A glyceroglycolipid lipase which is a protein of any one of the following a) to e):
- a) a protein consisting of the amino acid sequence of SEQ ID NO:
- 2 of the sequence listing;
- b) a protein consisting of an amino acid sequence encoded by the nucleotide sequence from nucleotide number 110 to nucleotide number 991 of SEQ ID NO:
- 1 of the sequence listing;
- c) a protein consisting of an amino acid sequence in which one or several amino acids are substituted, deleted, inserted, or added in the amino acid sequence as recited in a) or b), and having a glyceroglycolipid degradation activity;
- d) a protein consisting of an amino acid sequence having an amino acid sequence homology of 70% or more with the protein of a), and having a glyceroglycolipid degradation activity;
- e) a protein comprising the amino acid sequence as recited in a) or b).
DNA described in any one of the following a) to e):
- a) DNA consisting of the nucleotide sequence from nucleotide number 110 to nucleotide number 991 of SEQ ID NO:
- 1 of the sequence listing;
- b) DNA consisting of a nucleotide sequence having a nucleotide sequence homology of 70% or more with the DNA of a), and encoding a protein having a glyceroglycolipid degradation activity;
- c) DNA hybridizing to the DNA of a) under stringent conditions, and encoding a protein having a glyceroglycolipid degradation activity;
- d) DNA encoding a protein consisting of the amino acid sequence of SEQ ID NO:
- 2 of the sequence listing;
- e) DNA comprising the nucleotide sequence from nucleotide number 110 to nucleotide number 991 of SEQ ID NO:
- 1 of the sequence listing.
A method for producing a glyceroglycolipid lipase, comprising 1) and 2):
- 1) a step of culturing Aspergillus japonicus under conditions under which a glyceroglycolipid lipase is produced, and
- a step of separating and purifying the glyceroglycolipid lipase from the culture product of 1).
Dependencies are inferred from the claim text.