Foot and mouth disease virus recombinant vaccines and uses thereof
OwnerJean-Christophe Audonnet, Xuan Guo +4inventors
InventorsJean-Christophe Audonnet, Xuan Guo, Bradley J. Feilmeir, Karolyn Marie Troupe, Michel Bublot, Kevin Cox
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Abstract
The present invention encompasses FMDV vaccines or compositions. The vaccine or composition may be a vaccine or composition containing FMDV antigens. The invention also encompasses recombinant vectors encoding and expressing FMDV antigens, epitopes or immunogens which can be used to protect animals, in particular ovines, bovines, caprines, or porcines, against FMDV.
Claims21
A composition comprising an FMDV antigen and a pharmaceutical or veterinarily acceptable carrier, excipient, or vehicle.
A method of vaccinating a host susceptible to ovine, bovine, caprine, or porcine FMDV comprising a prime-boost administration protocol.
A substantially purified FMDV polypeptide expressed in a plant or microalga, wherein the polypeptide comprises:
- a) an amino acid sequence having at least 80% sequence identity to a polypeptide having the sequence as set forth in SEQ ID NOs:3, 10, 12, 17, 20, 23, 26, or 29;
- b) a conservative variant of the amino acid sequence as set forth in SEQ ID NOs:3, 10, 12, 17, 20, 23, 26, or 29;
- c) an immunogenic fragment comprising at least eight consecutive amino acids of the amino acid sequence as set forth in SEQ ID NOs:3, 10, 12, 17, 20, 23, 26, or 29, that specifically binds to an antibody that specifically binds to the amino acid sequence as set forth in SEQ ID NOs:3, 10, 12, 17, 20, 23, 26, or 29.
A plasmid comprising a DNA fragment having at least 70% sequence identity to the sequence as set forth in SEQ ID NO:1, 2, 4, 8, 9, 11, 13, 14, 15, 16, 18, 19, 21, 22, 24, 25, 27, 28, 30-35.
A stably transformed duckweed plant or microalga culture transformed with a gene expressing an FMDV antigen or fragment or variant thereof.
A method of producing an FMDV antigen comprising:
- (a) culturing within a duckweed culture medium a duckweed plant culture or a duckweed nodule culture, wherein the duckweed plant culture or the duckweed nodule culture is stably transformed to express the antigen, and wherein the antigen is expressed from a nucleotide sequence comprising a coding sequence for the antigen;
- and (b) collecting the antigen from the culture biomass.
Dependencies are inferred from the claim text.