Bacterial Polysaccharide-Polypeptide Conjugate Compositions

A composition comprising:

• at least one polysaccharide-polypeptide conjugate;
• at least one peptide comprising a sequence R1—XZXZNXZX—R2 (SEQ ID NOs:
• 1-5), whereby N is a whole number between 3 and 7, X is a positively charged natural and/or non-natural amino acid residue, each Z is independently an amino acid residue further defined as L, V, I, F and/or W, and R1 and R2 are independently —H, —NH2, —COCH3, —COH, a peptide with up to 20 amino acid residues or a peptide reactive group or a peptide linker with or without a peptide, X—R2 is an amide, ester or thioester of the C-terminal amino acid residue of the peptide (“Peptide A”);
• and
• at least one immunostimulatory oligodeoxynucleic acid molecule (ODN) having the structure of the formula (I)
• wherein:
• R1 is hypoxanthine or uracile;
• any X is O or S;
• any NMP is a 2′ deoxynucleoside monophosphate or monothiophosphate, further defined as deoxyadenosine-, deoxyguanosine-, deoxyinosine-, deoxycytosine-, deoxyuridine-, deoxythymidine-, 2-methyl-deoxyinosine-, 5-methyl-deoxycytosine-, deoxypseudouridine-, deoxyribosepurine-, 2-amino-deoxyribosepurine-, 6-S-deoxyguanine-, 2-dimethyl-deoxyguanosine- or N-isopentenyl-deoxyadenosine-monophosphate or -monothiophosphate;
• NUC is a 2′ deoxynucleoside, further defined as deoxyadenosine-, deoxyguanosine-, deoxyinosine-, deoxycytosine-, deoxyinosine-, deoxythymidine-, 2-methyl-deoxyuridine-, 5-methyl-deoxycytosine-, deoxypseudouridine-, deoxyribosepurine-, 2-amino-deoxyribosepurine-, 6-S-deoxyguanine-, 2-dimethyl-deoxyguanosine- or N-isopentenyl-deoxyadenosine;
• a and b are integers from 0 to 100 with the proviso that a+b is between 4 and 150;
• and
• B and E are common groups for 5′ or 3′ ends of nucleic acid molecules (“I-/U-ODN”).