Ligands that bind il-13
OwnerRudolf M. T. De Wildt, Inusha De Silva +1inventors
InventorsRudolf M. T. De Wildt, Inusha De Silva, Milan Ovecka
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Abstract
Disclosed are ligands that have binding specificity for interleukin-13 (IL-13), or for IL-4 and IL-13. Also disclosed are methods of using these ligands. In particular, the use of these ligands for treating IL-13-mediated conditions, such as lung conditions, eg allergic asthma, is described. The ligands have potent IL-13 binding kinetics. Ligands are described that are cross-reactive between human IL-13 and one or more primate IL-13. Ligands are well expressed in prokaryotic cells.
Claims43
An anti-interleukin-13 (IL-13) immunoglobulin single variable domain comprising an amino acid sequence that is identical to DOM10-53-474, with the exception that the amino acid sequence has 1, 2, 3, 4 or 5 amino acid changes compared to DOM10-53-474 (SEQ ID NO:
- 1) and wherein the single variable domain has a valine at position 28 according to Kabat numbering, wherein the single variable domain is not DOM10-53-616 (SEQ ID NO:
- 5).
An anti-interleukin-13 (IL-13) immunoglobulin single variable domain comprising an amino acid sequence that is identical to DOM10-53-474, with the exception that the amino acid sequence has 1, 2, 3, 4 or 5 amino acid changes compared to DOM10-53-474 (SEQ ID NO:
- 1) and wherein the single variable domain has the sequence XGX′X″, wherein the G is at position 54 according to Kabat numbering and
- X=H or K;
- X′=G or K;
- X″=K or I
- wherein the single variable domain is not DOM10-53-616 (SEQ ID NO:
- 5).
An anti-interleukin-13 (IL-13) immunoglobulin single variable domain, wherein the variable domain is DOM10-53-546 (SEQ ID NO:
- 2);
- DOM10-53-567 (SEQ ID NO:
- 3) or DOM10-53-568 (SEQ ID NO:
- 4).
An anti-interleukin-13 (IL-13) immunoglobulin single variable domain encoded by the nucleotide sequence of DOM10-53-546 (SEQ ID NO:
- 6);
- DOM10-53-567 (SEQ ID NO:
- 7) or DOM10-53-568 (SEQ ID NO:
- 8).
An isolated or recombinant nucleic acid, wherein the nucleic acid comprises a nucleotide sequence that is at least 99% identical to the nucleotide sequence of DOM10-53-546 (SEQ ID NO:
- 6);
- DOM10-53-567 (SEQ ID NO:
- 7);
- DOM10-53-568 (SEQ ID NO:
- 8);
- or DOM10-53-616 (SEQ ID NO:
- 9), and wherein the nucleic acid encodes a polypeptide comprising an immunoglobulin single variable domain that specifically binds to IL-13.
Dependencies are inferred from the claim text.