System and method for producing t cells
OwnerLung-Ji Chang, Ekta Samir Patelinventors
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Abstract
Disclosed herein is a system and method for producing T cells from stem cell populations. Specifically exemplified herein is a culture system and method that produces CD4 cells and/or T cell subtypes from a CD4 lineage using a sample of hematopoietic stem cells. Adult hematopoietic precursor/stem cells (HPC) are progenitors to all lineages of immune cells. There has been limited success in generating functional CD4 T cells with this convenient culture system. Also disclosed herein is a novel stromal cell line expressing DL1, interleukin-7 (IL-7), and FMS-like tyrosine kinase 3 ligand (Flt3-L). This improved culture system can greatly facilitate the study of late T cell development and enables immunotherapeutic applications.
Claims14
A method of producing fully mature and functional CD4 T cells from hematopoietic stem cells (HSCs) wherein the method comprises culturing the HSCs under culture conditions to direct development of said HSCs to the functional CD4 T cells, the culture conditions comprising:
- culturing the HSCs in the presence of IL-7 for at least 2 weeks, and
- terminating subjection of said stem cells to IL-7 at a time somewhere between about 2 weeks to about 4 weeks
A pharmaceutical composition comprising functional CD4 T cells cultured and produced from adult human bone marrow and a pharmaceutically acceptable carrier, excipient, or diluent.
An isolated cell sample of modified fetal stromal cells engineered to express delta-like 1 ligand and IL-7 and/or Flt31.
Dependencies are inferred from the claim text.