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US 2011/0236356 A1Published application
Methods of isolating and using stem cells
AssigneesNova Southeastern University, University of Miami
InventorsC.-Y. Charles Huang, Franklin Garcia-Godoy, Herman S. Cheung
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Abstract
Methods of isolating and using stem cells from neural crest, e.g. periodontal ligament, isolated stem cell and therapeutic cell cultures, and therapeutic applications for a variety of conditions. The cells and cell cultures are especially useful for autologous administration.
Claims18
A method for isolating stem cells comprising:
- providing an initial cell culture of tissue derived from the cranial neural crest;
- screening the initial cell culture for those cells expressing a marker of interest associated with stem cells;
- and isolating the cells expressing the stem cell marker of interest to obtain an isolated stem cell culture
A method of producing isolated therapeutic cells comprising:
- a) screening a culture of periodontal ligament cells for those cells expressing a marker of interest associated with stem cells;
- b) isolating the cells expressing the marker of interest;
- and
- c) differentiating the isolated cells into therapeutic cells.
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Dependencies are inferred from the claim text.