Methods to treat or prevent a skin condition using a nell1 peptide
AssigneeNellone Therapeutics, Inc.
InventorsDora P. Mitchell, Cymbeline T. Culiat, Beverly W. Lubit
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Abstract
The described invention provides methods and compositions utilizing a NELL1 peptide or nucleic acid encoding a NELL1 peptide for treating or preventing a skin condition. The methods include treating or preventing manifestations of aging in human skin, repairing damage to skin, and preventing skin scarring. Methods are also provided for assaying a test peptide for NELL1 activity.
Claims40
A method for treating or preventing a skin condition, the method comprising administering a NELL1 peptide to a subject in need thereof, whereby at least one manifestation of the skin condition is treated or prevented.
A method for assaying a test peptide for NELL1 activity, said method comprising the steps of:
- a) administering said test peptide to a skin sample;
- b) irradiating said skin sample with ultraviolet light;
- c) assessing the expression level of an inflammatory mediator, a matrix metalloproteinase, or an aquaporin in the skin sample;
- wherein step a) is performed at least one of before, during, and after step b);
- and
- wherein a reduction in UV-stimulated expression of an inflammatory mediator or matrix metalloproteinase is indicative of NELL1 activity;
- or
- wherein an increase in UV-stimulated aquaporin expression is indicative of NELL1 activity.
A method for assaying a test peptide for NELL1 activity, said method comprising the steps of:
- a) administering said test peptide to a skin sample;
- b) irradiating said skin sample with ultraviolet light;
- c) assessing the number of sunburned cells in said skin sample;
- wherein step a) is performed at least one of before, during, and after step b);
- and
- wherein a reduction in the number of sunburned cells as compared to a control sample irradiated with ultraviolet light is indicative of NELL1 activity.
A method for treating or preventing a skin condition, the method comprising administering a heterologous nucleic acid molecule having a nucleotide sequence encoding a NELL1 peptide to a subject in need thereof, whereby at least one manifestation of the skin condition is treated or prevented.
Dependencies are inferred from the claim text.