Manipulation of Organic Acid Biosynthesis and Secretion
AssigneeAgriculture Victoria Services PTY LTD
InventorsGerman Spangenberg, Michael Emmerling, Eng Kok Ong, Ramiro Mendez, Stephen Panter, Marcel Labandera
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Abstract
The present invention relates to nucleic acid fragments encoding amino acid sequences for organic acid biosynthetic enzymes in plants, and the use thereof for the modification of, for example, organic acid biosynthesis and secretion in plants. In particularly preferred embodiments, the invention relates to the combinatorial expression of citrate synthase (CS) and/or malate dehydrogenase (MDH) and/or phosphoenolpyruvate carboxylase (PEPC) in plants to modify, for example, organic acid synthesis and secretion.
Claims21
1-29. (canceled)
A substantially purified or isolated nucleic acid or nucleic acid or nucleic acid fragment encoding an organic acid biosynthesis enzyme polypeptide selected from the group consisting of a citrate synthase (CS) polypeptide, a phosphoenolpyruvate carboxylase (PEPC) polypeptide, or a functionally active fragment or variant of such a polypeptide, from a clover (Trifolium), medic (Medicago), ryegrass (Lolium) or fescue (Festuca) species.
A nucleic acid or nucleic acid fragment encoding a CS polypeptide and comprising a nucleotide sequence selected from the group consisting of:
- (a) sequences shown in SEQ ID NOS 1, 3 to 10, 11, 13 to 16, 17, 19, 327, 329 to 335, 336, 338 to 344, 349, 351 and 353;
- (b) complements of the sequences recited in (a);
- (c) sequences antisense to the sequences recited in (a) and (b);
- (d) functionally active fragments and variants of the sequences recited in (a), (b) and (c);
- and
- (e) RNA sequences corresponding to the sequences recited in (a), (b), (c) and (d).
A nucleic acid or nucleic acid fragment encoding a PEPC polypeptide and including a nucleotide sequence selected from the group consisting of:
- (a) sequences shown in SEQ ID NOS 187, 189, 191 to 197, 199, 201, 203, 310, 312 to 314, 315, 317 to 318, 319, 321 to 322, 323, 325 and 347;
- (b) complements of the sequences recited in (a);
- (c) sequences antisense to the sequences recited in (a) and (b);
- (d) functionally active fragments and variants of the sequences recited in (a), (b) and (c);
- and
- (e) RNA sequences corresponding to the sequences recited in (a), (b), (c) and (d).
A substantially purified or isolated polypeptide from a clover (Trifolium), medic (Medicago), ryegrass (Lolium), or fescue (Festuca) species, selected from the group consisting of CS and PEPC polypeptides;
- and functionally active fragments and variants thereof.
Dependencies are inferred from the claim text.