Sequence-characterized amplified region (scar) markers for plant resistance against fungal pathogens

A method for screening a plurality of plant cultivars for identification of individual cultivars resistant to infection by Leptosphaeria maculans, said method comprising the steps of:

• collecting from each individual cultivar comprising the plurality of cultivars, a biomass sample;
• preparing a nucleic acid preparation from each of said biomass samples;
• contacting each of said nucleic acid preparations with at least one of a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 1, a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 2, a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 20, and a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 21, and intermixing therewith;
• conducting an amplification reaction therewith each of said intermixed nucleic acid preparations;
• and
• assessing each of said amplified nucleic acid preparations to identify individual nucleic acid preparations wherein a hybridization reaction with one of said nucleotide sequences occurred during the amplication reaction, wherein a hybridization reaction is indicative of the cultivar's resistance to infection by Leptosphaeria maculans.

A method for screening a plurality of plant cultivars for identification of individual cultivars containing therein Rpg3Dun gene sequences, said method comprising the steps of:

• collecting from each individual cultivar comprising the plurality of cultivars, a biomass sample;
• preparing a nucleic acid preparation from each of said biomass samples;
• contacting each of said nucleic acid preparations with at least one of a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 1, a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 2, a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 20, and a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 21, and intermixing therewith;
• conducting an amplification reaction therewith each of said intermixed nucleic acid preparations;
• and
• assessing each of said amplified nucleic acid preparations to identify individual nucleic acid preparations wherein a hybridization reaction with one of said nucleotide sequences occurred during the amplication reaction, wherein a hybridization reaction is indicative of the cultivar's containing therein Rpg3Dun gene sequences.

A gene cassette comprising at least one nucleotide sequence selected from the group consisting of a nucleotide sequence set forth in SEQ ID NO:

• 2, a nucleotide sequence set forth in SEQ ID NO:
• 2, a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 20, and a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 21.

A kit comprising a nucleotide sequence selected from the group consisting of a nucleotide sequence set forth in SEQ ID NO:

• 1, a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 2, a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 20, and a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
• 21, said nucleotide sequence complementary to a Rpg3Dun gene sequence.