Method for Detecting The Impacts of Interfering Effects on Experimental Data
AssigneeEvotec OAI AG
InventorsChristian Eggeling, Kaupo Palo, Nicolas Fay, Maciej Hoffman-Wecker, Pierre Ilouga
Life of this patent
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Abstract
The invention provides a method for identifying the impacts of interfering effects on experimental data. In particular, a method is described for identifying the impacts of unwanted auto-fluorescence, fluorescence quenching, and deterioration of a fluorescent sample under study on the collected experimental data. The data are analyzed whether or not said data fulfill certain criteria with respect to a threshold which is indicative for said interfering effect.
Claims11
1-36. (canceled)
A system for detecting the impact of interfering effects on experimental data resulting from optical experiments, said system comprising:
- (i) means for supporting one or a plurality of samples in an inspection station (2),
- (ii) one or a plurality of photosensitive detectors (9, 10) which are positioned relative to the inspection station (2) so that electromagnetic radiation emitted from the samples impinges on the detectors (9, 10), and a
- (iii) signal processing unit (11) comprising
- means for addressing the photosensitive detectors (9, 10) to generate experimental data,
- means for determining values of one or a plurality of identification parameters from said data,
- means for storing the values in such a manner that preferably all the values which relate to any one of the samples are linked,
- means for creating a multi-dimensional histogram or distribution of the values of the identification parameters,
- means for determining one or a plurality of thresholds for the values of identification parameters from said histogram or distribution, which thresholds are indicative for the interfering effects, wherein the threshold for the values of a first identification parameter is a function of a corresponding second identification parameter,
- means for analyzing the values of the one or a plurality of identification parameters whether or not these fulfill one or a plurality of criteria with respect to the thresholds, and
- means for supplying as output information the experimental data influenced and/or the experimental data are not affected by the interfering effects.
A signal processing unit (11) for processing experimental data which may be deteriorated by interfering effects comprising
- means for storing experimental data,
- means for determining values of one or a plurality of identification parameters from said data,
- means for storing the values in such a manner that all the values which relate to any one of the samples are linked,
- means for creating a multi-dimensional histogram or distribution of the values of the identification parameters,
- means for determining one or a plurality of thresholds for the values of identification parameters from said histogram or distribution, which thresholds are indicative for the interfering effects, wherein the threshold for the values of a first identification parameter is a function of a corresponding second identification parameter,
- means for analyzing the values of one or a plurality of the identification parameters whether or not these fulfill one or a plurality of criteria with respect to the thresholds, and
- means for supplying as output information the experimental data influenced and/or the experimental data not affected by the interfering effects.
(canceled)
Dependencies are inferred from the claim text.