Polypeptides and methods for producing triacylglycerols comprising modified fatty acids
AssigneesCommonwealth Scientific and Industrial Research Organisation, Grains Research and Development Corporation
InventorsXue-Rong Zhou, Surinder Pal Singh, Allan Green
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Abstract
The present invention relates to methods of producing modified fatty acids comprising a functional group which is a hydroxyl group, an epoxy group, an acetylenic group or a conjugated double bond. For example, seeds, seedoil and methods of making seedoil are provided wherein at least 23% (mol %) of the fatty acid content of the seed or seedoil comprises the functional group. Also provided are novel polypeptides, and polynucleotides thereof, which can be used to produce the modified fatty acids, particularly in transgenic plants and cells suitable for fermentation.
Claims10
1-61. (canceled)
A process for producing seedoil, comprising the steps of
- i) obtaining a transgenic seed having one or more epoxygenated fatty acids in its seedoil such that at least 23% of the fatty acid content of the seedoil comprises an epoxy group, wherein the seed is from Brassica sp., Gossypium hirsutum, Linum usitatissimum, Helianthus sp., Carthamus tinctorius, Glycine max, Zea mays or Arabidopsis thaliana, and wherein the seedoil has one or more of
- a) less than 4% (mol %) of the total fatty acid content of the seedoil is linolenic acid,
- b) at least 10% (mol %) of fatty acids esterified at the sn-3 position of total triacylglycerols in the seedoil comprises the epoxy group,
- c) at least 10% (mol %) of fatty acids esterified at the sn-2 position of total triacylglycerols in the seedoil comprises the epoxy group,
- d) at least 10% (mol %) of fatty acids esterified at the sn-1 position of total triacylglycerols in the seedoil comprises the epoxy group,
- e) at least 10% of the seedoil is bi-vernoleate, or
- f) at least 4% of the seedoil is tri-vernoleate, and
- ii) processing the seed to extract the seedoil.
Seedoil comprising one or more epoxygenated fatty acids such that at least 28% (mol %) of the fatty acid content of the seedoil comprises an epoxy group, wherein the seedoil is from Brassica sp., Gossypium hirsutum, Linum usitatissimum, Helianthus sp., Carthamus tinctorius, Glycine max, Zea mays or Arabidopsis thaliana and wherein the seedoil has one or more of
- a) less than 4% (mol %) of the total fatty acid content of the seedoil is linolenic acid,
- b) at least 10% (mol %) of fatty acids esterified at the sn-3 position of total triacylglycerols in the seedoil comprises the epoxy group,
- c) at least 10% (mol %) of fatty acids esterified at the sn-2 position of total triacylglycerols in the seedoil comprises the epoxy group,
- d) at least 10% (mol %) of fatty acids esterified at the sn-1 position of total triacylglycerols in the seedoil comprises the epoxy group,
- e) at least 10% of the seedoil is bi-vernoleate, or
- f) at least 4% of the seedoil is tri-vernoleate.
Dependencies are inferred from the claim text.