Eif2gamma gene as a diagnostic target for the identification of fungal and yeast species
AssigneeNational University of Ireland, Galway
InventorsThomas Gerard Barry, Majella Maher, Terry James Smith, Marcin Jankiewicz, Louise O'Connor, Nina Tuite, Sinead Lahiff
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Abstract
The current invention relates to a diagnostic kit for a yeast or fungal species comprising at least one oligonucleotide probe capable of binding to at least a portion of the eIF2y gene or its corresponding mRNA.
Claims21
A diagnostic kit for a yeast or fungal species comprising at least one oligonucleotide probe capable of binding to at least a portion of the eIF2 γ gene or its corresponding mRNA.
A kit as claimed in any preceding claim, further comprising at least one primer for amplification of at least a portion of the eIF2 γ gene.
A kit as claimed in any preceding claim, comprising at least one forward and at least one reverse primer for at least a portion of the eIF2 γ gene
A kit as claimed in any preceding claim, comprising at least one forward in vitro amplification primer and at least one reverse in vitro amplification primer, the forward amplification primer being selected from the group comprising SEQ ID NO 3, 5, 89, 91, 93, 95, 97, 104, 105, 112, 113, 114, 115, 116, 117, 118, 119, 120, 135 or 136, or sequences substantially similar or complementary thereto which can also act as a forward amplification primer for the eIF2 γ gene and the reverse amplification primer being selected from the group comprising SEQ ED NO 4, 6, 90, 92, 94, 96, 98, 106, 107, 121, 122, 123, 124, or 137, or sequences substantially similar or complementary thereto which can also act as a reverse amplification primer for the elF2 γ gene.
A nucleic acid molecule selected from the group consisting of:
- SEQ ID NO 1 through SEQ ID NO 73 and SEQ ID NO. 84 to 135 and sequences substantially homologous or substantially complementary thereto or to a portion thereof and having a function in diagnostics based on the eIF2 γ gene.
A nucleic acid molecule comprising an oligonucleotide having a sequence substantially homologous to or substantially complementary to a portion of a nucleic acid molecule as claimed in claim 10.
A method of detecting a target organism in a test sample comprising the steps of
- (i) Mixing the test sample with at least one oligonucleotide probe capable of binding to at least a portion of the eIF2 γ gene or its corresponding mRNA under appropriate conditions,
- (ii) hybridizing under high stringency conditions any nucleic acid that may be present in the test sample with the oligonucleotide to form a probe:target duplex;
- and
- (iii) determining whether a probe:target duplex is present;
- the presence of the duplex positively identifying the presence of the target organism in the test sample
A kit substantially as described herein with reference to the accompanying figures.
A nucleic acid substantially as described herein with reference to the accompanying figures.
A method substantially as described herein with reference to the accompanying figures.
Dependencies are inferred from the claim text.