Enhanced Immunosorbent Spot Test Device and Method of Using Same
OwnerArnold J. AQUILINOinventor
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Abstract
A device used for immunosorbent spot testing. The present invention makes use of a membrane and carrier to test for a multiplex of analytes and is read visually without the use of an optical reader. The membrane comprises of a plurality of specific analyte detectors which each contain a calibrated antigen. The calibrated antigen comprises of a plurality of binding sites which bind a plurality of specific analyte to be tested for. The bound specific analyte is marked with conjugate and further bound with chromogen to provide a visual indiciator as a result of presence of the specific analyte.
Claims11
An apparatus for an enhanced immunosorbent spot test device comprises,
- a membrane;
- a carrier;
- the membrane comprising a plurality of specific analyte detectors and a control spot;
- the plurality of specific analyte detectors comprising of a plurality of binding sites, a plurality of blockers, a wash buffer, a plurality of specific analyte, a first conjugate, a second conjugate, a chromogen, a wash buffer, and a specimen;
- and
- the plurality of specific analyte detectors being evenly spaced along the membrane.
A method of use for an enhanced immunosorbent spot test device comprises,
- providing a wash buffer concentrate;
- combining the wash buffer concentrate with 500 ml of distilled water to create a wash buffer;
- combining 2 ml of the wash buffer with 20 μl of a concentrated specimen to create a specimen;
Dependencies are inferred from the claim text.