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US 2011/0217791 A1Published application
Method for quantifying or detecting dna
AssigneeSumitomo Chemical Company, Limited
InventorsYoshitaka Tomigahara, Hideo Satoh, Hirokazu Tarui
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Abstract
The present invention relates to a method for quantifying or detecting DNA having a target DNA region, and so on.
Claims14
A method for quantifying or detecting DNA which comprises a target DNA region and is contained in a specimen, comprising:
- (1) A first step of preparing a specimen containing a test oligonucleotide that is DNA comprising a target DNA region;
- (2) A second step of mixing the test oligonucleotide contained in the specimen prepared in the first step and a detection oligonucleotide that is capable of complementarily binding with the test oligonucleotide and has a plurality of identification functions, to form a detection complex comprising the test oligonucleotide and the detection oligonucleotide, and immobilizing the detection complex to a support;
- and
- (3) A third step of quantifying or detecting said DNA comprising a target DNA region by detecting the detection oligonucleotide contained in the detection complex formed in the second step by its identification function.
1 more element
A method for quantifying or detecting DNA which comprises a target DNA region and is contained in a specimen, comprising:
- (1) A first step of preparing a specimen containing a test oligonucleotide that is DNA comprising a target DNA region,
- (2) A second step of mixing the test oligonucleotide contained in the specimen prepared in the first step, and a detection oligonucleotide that is capable of complementarily binding with the test oligonucleotide and comprises a methylated oligonucleotide, to form a detection complex comprising the test oligonucleotide and the detection oligonucleotide, and immobilizing the detection complex to a support, and
- (3) A third step of quantifying or detecting said DNA comprising a target DNA region by detecting the detection oligonucleotide contained in the detection complex formed in the second step by its identification function.
Dependencies are inferred from the claim text.