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AssigneeUniversity of Maryland, College Park
InventorsYi Liu, Xiao-Wen Shi, Gregory F. Payne, W. Lee Meyer
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A method of forming a bioelectronic device including a protein on an electrically conductive substrate, by electro-depositing aminopolysaccharide chitosan on the substrate while applying a cathodic voltage to the substrate, to form an aminopolysaccharide chitosan film thereon, applying an anodic voltage to the substrate in the presence of NaCl to activate the aminopolysaccharide chitosan film so that it is reactive with protein. The method also optionally includes reacting the aminopolysaccharide film, after activation thereof, with the protein, so that the protein assembles on and is coupled to the substrate, thereby forming a bioelectronic device. The protein can include single or multiple protein species, and including biosensing proteins. Additional methods include biosensing of electrochemically active compounds either present in a sample or generated during a biological recognition event and devices useful in such methods. The resulting devices are useful as sensors in hand-held devices, textiles, garments and the like.
A method of forming a bioelectronic device including a protein on an electrically conductive substrate, comprising:
(canceled)
6-10. (canceled)
A method of forming an electrically conductive substrate adapted for assembly of a protein species thereon, said method comprising:
A bioelectronic device, comprising:
18-35. (canceled)
A method of detecting the presence of a protein in a sample comprising the steps of:
A method of transducing enzyme substrate recognition into an electrical signal comprising the steps of:
Dependencies are inferred from the claim text.