Leader Sequence to Boost Gene Expression
AssigneeThe Trustees of the University of Pennsylvania
InventorsJoshua B. Plotkin, Grzegorz Kudla
Life of this patent
Estimating the term…
Abstract
The invention provides compositions and methods for enhanced gene expression. The invention provides a composition comprising a 28-codon leader sequence operably linked to a desired gene which encodes the desired protein.
Claims7
An isolated nucleic acid molecule comprising SEQ ID NO:
- 12.
An isolated nucleic acid molecule comprising SEQ ID NO:
- 12 operably linked to a coding sequence.
An expression cassette for expressing a gene in a cell, wherein said cassette comprises operably linked elements comprising a promoter, a leader sequence, and a coding sequence, wherein said leader sequence comprises SEQ ID NO:
- 12.
A cell comprising an expression cassette, wherein said cassette comprises operably linked elements comprising a promoter, a leader sequence, and a coding sequence, wherein said leader sequence comprises SEQ ID NO:
- 12.
A method for enhancing expression of a gene in a cell, the method comprising expressing an expression cassette in a cell, wherein said cassette comprises operably linked elements comprising a promoter, a leader sequence, and a coding sequence, wherein said leader sequence comprises SEQ ID NO:
- 12.
A kit for enhancing expression of a gene in a cell, the kit comprising an isolated nucleic acid molecule comprising SEQ ID NO:
- 12, the kit further comprising an instructional material for the use thereof.
Dependencies are inferred from the claim text.