Loading patent…
AssigneeTaipei Medical University
InventorsYu-Hui Tsai, Li-Hsuan Chiu, Chin-Chean Wong, Wen-Fu Lai, Shih-Ching Chen
Estimating the term…
The present invention provides a method for restoring native chondrocyte phenotype and functions of, and/or increasing type II collagen as well as aggrecan mRNA expression levels and GAG accumulation level in dedifferentiated chondrocytes which have been subcultured and expanded in vitro, which comprising culturing the said dedifferentiated chondrocytes in vitro with a medium comprising type II collagen, or its biologically active peptide fragment(s) or analogs with or without growth factor(s), wherein the type II collagen or its biologically active peptide fragment(s) or analogs are effective to restore chondrocyte phenotype and functions of, and/or to increase type II collagen and aggrecan expression levels and GAG accumulation level in the said dedifferentiated chondrocytes.
A method for restoring native chondrocyte phenotype and functions of, and/or increasing type II collagen and AGN expression levels and GAG accumulation level in dedifferentiated chondrocytes which have been subcultured and expanded in vitro, which comprising culturing the said dedifferentiated chondrocytes in vitro with a medium comprising type II collagen, or its biologically active peptide fragment(s), or analogs effective to restore native chondrocyte phenotype and functions of, and/or increasing type II collagen and AGN expression levels and GAG accumulation level in dedifferentiated chondrocytes.
A medium for restoring native chondrocyte phenotype and functions of, and/or increasing type II collagen and AGN expression levels and GAG accumulation level in the dedifferentiated chondrocytes which have been subcultured and expanded in vitro, comprising a medium used to subculture and expand chondrocytes and type II collagen, its biologically active peptide fragment(s), or analogs in an effective amount.
Dependencies are inferred from the claim text.