Animal protein free media for cultivation of cells
AssigneesBaxter International Inc., Baxter Healthcare S.A.
InventorsManfred Reiter, Wolfgang Mundt, Leopold Grillberger, Barbara Kraus
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Abstract
The present invention relates to animal protein free cell culture media comprising a combination of non-animal derived peptides derived from soy hydrolysate and yeast hydrolysate. The invention also provides an animal protein free culture process, wherein cells are cultivated, propagated and passaged without animal-derived components. This process is useful for cultivating cells, such as recombinant cells or cells infected with a virus, and for production biological products by cell culture processes under conditions devoid of animal protein components.
Claims16
A method of cultivating mammalian cells to obtain a confluent cell culture, comprising:
- providing an animal protein-free medium comprising soy hydrolysate and yeast hydrolysate;
- growing the cells in the medium;
- and
- passaging and sub-cultivating the cells grown in the medium while in contact with a non-animal protease in order to obtain a confluent cell culture.
Dependencies are inferred from the claim text.