US 2011/0217770 A1 · Cell Piercing Device and Method of Using Same

Abstract

A cell piercing device comprising a container, a respectively matching cap and a cell interacting surface is disclosed. The cell interacting surface comprises a plurality of extensions or prongs. The cell piercing device may further comprise a pedestal. Furthermore, a method of facilitating an interaction with the interior of cells is disclosed. The method includes the steps of loading into the container of the cell piercing device, a liquid solution containing the cells and tightening the respectively matching cap. Then centrifuging the device while the extensions or prongs protruding from the cell interacting surface are oriented essentially within the direction of the vector of the centripetal force and thereafter centrifuging the device while said extensions or prongs are oriented essentially within the direction of the vector of the centrifugal force.

Claims14

Dependent claims

A cell piercing device comprising:

a container comprising an anterior opening allowing loading and discharging of solutions and a bottom;
a respectively matching cap, capable of sealing said container;
at least one cell interacting surface, disposed in said container, said cell interacting surface comprising a plurality of extensions or prongs, and
a pedestal, spaced apart from said bottom of said container.

1 more element

A method of facilitating an interaction with the interior of cells, said method comprising the steps of:

loading a cell piercing device into a container, through an anterior opening thereof, a liquid solution containing said cells;
tightening a respectively matching cap, thereby sealing said container;
centrifuging said device while extensions or prongs protruding from a cell 1 interacting surface, disposed in said container, are oriented essentially within the direction of the vector of the centripetal force;
whereby said cells are urged by 1 the centrifugal force towards said cell interacting surface and consequently pierced by said extensions or prongs and spitted onto them;
centrifuging said device while said extensions or prongs are oriented essentially within the direction of the vector of the centrifugal force;
whereby said cells are urged by the centrifugal force away from said cell interacting surface and consequently driven off said extensions or prongs and released into the interior of said container;
removing said cap from said container and collecting said cells.

4 more elements

Dependencies are inferred from the claim text.

Cell Piercing Device and Method of Using Same

Life of this patent

Cell Piercing Device and Method of Using Same

Life of this patent

Abstract

Claims14