Methods of Producing Peptides in Plants and Peptides Produced Thereby
AssigneeOhio University
InventorsMarcia J. Kieliszewski, Jianfeng Xu, Gary D. Meyer
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Abstract
Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion are present. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are also present.
Claims16
A nucleic acid construct for expression of at least one biologically active protein in plants comprising:
- a) at least one nucleic acid sequence encoding a plant hydroxyproline O-glycosylation site b) at least one nucleic acid sequence encoding a biologically active vertebrate protein and c) an operatively linked plant promoter;
- wherein the at least one biologically active protein in plants exhibits a pharmacokinetic plasma half-life that is increased as compared with the same biologically active protein in plants that lacks a plant hydroxyproline O-glycosylation site.
A nucleic acid construct for expression of at least one biologically active protein in plants comprising:
- a) at least one nucleic acid encoding at least one hydroxyproline O-glycosylation plant glycomodule, b) at least one nucleic acid sequence encoding a biologically active vertebrate protein, and c) an operatively linked plant promoter;
- wherein the at least one biologically active protein in plants exhibits a pharmacokinetic plasma half-life that is increased as compared with the same biologically active protein in plants that lacks at least one hydroxyproline O-glycosylation plant glycomodule.
Dependencies are inferred from the claim text.