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AssigneeLife Technologies Corporation
InventorsJun E. Lee, Ayoub Rashtchian
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The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.
1-19. (canceled)
The method of claim 47, wherein said mixture further comprises one or more nucleotides.
The method of claim 47, wherein said mixture further comprises one or more oligonucleotide primers.
24-26. (canceled)
The method of claim 47, wherein said reverse transcriptase is substantially reduced in RNase H activity.
The method of claim 47, wherein said one or more DNA polymerases are thermostable DNA polymerases.
29-46. (canceled)
A method for amplifying a nucleic acid molecule comprising:
48-55. (canceled)
A composition comprising a Moloney murine leukemia virus (M-MLV) reverse transcriptase and one or more DNA polymerases, wherein the unit ratio of said reverse transcriptase to said DNA polymerases is greater than about 3:2.
A method for one-step RT-PCR, said method comprising:
Dependencies are inferred from the claim text.