Compositions and methods for the synthesis of appa-containing peptides
AssigneeUniversity of Illinois at Urbana-Champaign
InventorsWilliam W. Metcalf, Wilfred A. van der Donk, Junkal Zhang, Benajmin T. Circello, Svetlana A. Borisova
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Abstract
The disclosure of the present application provides polypeptide sequences and nucleotide sequences coding for the polypeptide sequences of proteins used in the production of APPA-containing peptides. In at least one embodiment of the present disclosure, an isolated nucleic acid is disclosed which comprises a nucleotide sequence encoding a polypeptide having a sequence identity of 60 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS: 2-13, and 15-23.
Claims22
An isolated nucleic acid comprising a first nucleotide sequence encoding a first polypeptide having a first sequence identity of at least about 70 percent to an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 2-13 and 15-23.
A vector comprising an isolated nucleic acid comprising a first nucleotide sequence encoding a first polypeptide having a first sequence identity of 70 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 2-13 and 15-23.
A transformed cell comprising:
- a vector comprising an isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide having a sequence identity of 70 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 2-13 and 15-23;
- wherein the transformed cell is capable of producing an APPA-containing peptide.
A method for producing an APPA-containing peptide, the method comprises the steps of:
- transforming a host cell with an isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide having a sequence identity of 70 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 2-13, and 15-25;
- and
- growing the transformed host cell under conditions to produce an APPA-containing peptide.
Dependencies are inferred from the claim text.