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AssigneeThe Rockefeller University
InventorsBrian T. Chait, Alan J. Tackett, Jeffrey A. DeGrasse, Marlene Oeffinger, Michael P. Rout, Matthew D. Sekedat
Estimating the term…
The present invention is method of determining whether or not associations between a given protein and other proteins in a cell are specific. The method comprises (a) providing a first sample of the cells in which the given protein contains a tag, (b) providing a second sample of the same cells, wherein the given protein and the other proteins are metabolically labeled, and wherein neither the given protein nor the other proteins are tagged, (c) mixing and lysing the first cell sample and the second cell sample to provide a mixture of proteins, (d) binding the tag of the given protein to a substrate, (e) isolating proteins associated with the tagged given protein bound to the substrate, whereby the associated proteins comprise: (i) proteins specifically associated with the tagged given protein, (ii) proteins non-specifically associated with the tagged given protein, or (iii) a combination thereof, (f) determining whether each associated protein is unlabeled or a mixture of labeled and unlabeled proteins, wherein if the associated protein is not labeled, then that protein was specifically associated in the cell with the tagged given protein.
A method of determining whether or not associations between a given protein and other proteins in a cell are specific, the method comprising:
A test kit for determining whether an association between a given protein and other proteins in a cell is specific or non-specific comprising a growth medium with a metabolic label and cells containing the given protein and the other proteins.
-42. (canceled)
A kit, comprising a first cell sample and a container comprising a second cell sample, wherein the first cell sample and the second cell sample are of a same cell type, wherein the first cell sample comprises heavy-isotope labeled proteins, and wherein the ratio of cells in the first cell sample to cells in the second cell sample is known and is equal to or less than 100:1.
Dependencies are inferred from the claim text.