Lepa/guf1 gene sequences as a diagnostic target for the identification of bacterial species
AssigneeNational University of Ireland, Galway
InventorsThomas Gerard Barry, Majella Maher, Terence James Smith, Justin O'Grady
Life of this patent
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Abstract
The current invention relates to a diagnostic kit for a bacterial species and/or fungal and/or yeast species comprising at least one oligonucleotide probe capable of binding to at least a portion of the LepA and/or Guf1 genes or its corresponding mRNA.
Claims20
A diagnostic kit for a bacterial species and/or fungal and/or yeast species comprising at least one oligonucleotide probe capable of binding to at least a portion of the LepA and/or Guf1 genes or its corresponding mRNA.
A nucleic acid molecule selected from the group consisting of:
- SEQ ID NO 1 through SEQ ID NO 178 and sequences substantially homologous or substantially complementary thereto or to a portion thereof and having a function in diagnostics based on the LepA or Guf1 gene.
A method of detecting a target organism in a test sample comprising the steps of:
- (i) Mixing the test sample with at least one an oligonucleotide probe capable of binding to at least a portion of the LepA or Guf1 gene or its corresponding mRNA under appropriate conditions;
- (ii) hybridizing under a high stringency conditions any nucleic acid that may be present in the test sample with the oligonucleotide to form a probe:target duplex;
- and
- (iii) determining whether a probe:target duplex is present;
- the presence of the duplex positively identifying the presence of the target organism in the test sample.
20-22. (canceled)
Dependencies are inferred from the claim text.