P2/p2a/p2b gene sequences as diagnostic targets for the identification of fungal and yeast species
AssigneeNational University of Ireland, Galway
InventorsThomas Gerard Barry, Terry James Smith, Marcin Jankiewicz, Louise O'Connor, Nina Tuite, Sinead Lahiff, Majella Maher
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Abstract
The present invention relates to nucleic acid primers and probes to detect one or more fungal and yeast species. More specifically the invention relates to the P2, P2A and P2B gene sequences (also known as 60S acidic ribosomal protein P2, RLA-2-ASPFU, Allergen ASP f8 or Afp2), the corresponding RNA, specific probes, primers and oligonucleotides related thereto and their use in diagnostic assays to detect and/or discriminate fungal and yeast species.
Claims20
A diagnostic kit for a yeast or fungal species comprising an oligonucleotide probe capable of binding to at least a portion of the P2, P2A or P2B genes or its corresponding mRNA.
A nucleic acid molecule selected from the group consisting of:
- SEQ ID NO 1 through SEQ ID NO 110 and sequences substantially homologous or substantially complementary thereto or to a portion thereof and having a function in diagnostics based on the P2, P2A and/or P2B genes.
A method of detecting a target organism in a test sample comprising the steps of:
- (i) Mixing the test sample with at least one oligonucleotide probe capable of binding to at least a portion of the P2, P2A and/or P2B gene or its corresponding mRNA under appropriate conditions;
- (ii) hybridizing under a high stringency conditions any nucleic acid that may be present in the test sample with the oligonucleotide to form a probe:target duplex;
- and
- (iii) determining whether a probe:target duplex is present;
- the presence of the duplex positively identifying the presence of the target organism in the test sample.
23-25. (canceled)
Dependencies are inferred from the claim text.