BETAG1-IgG INTRON FOR ENHANCED ANTI-IGF1R EXPRESSON
AssigneeSchering Corporation
InventorDeba P. Saha
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Abstract
The present invention provides polynucleotides for enhanced expression of a target gene such as an immunoglobulin. Methods of expressing a target gene using the polynucleotides of the invention are also covered.
Claims50
An isolated polynucleotide comprising a beta-globin splice donor site and an immunoglobulin gamma splice acceptor site wherein said sites are separated by about 125 nucleotides;
- which is operably linked to a promoter, which sites and promoter are operably linked to a gene encoding an anti-IGF1R, anti-IL-23 p19, anti-IL23 receptor, anti-IL-17, anti-PD1 or anti-HGF antibody immunoglobulin light chain or heavy chain which chains are optionally fused to an immunoglobulin constant region.
An isolated plasmid vector characterized by a plasmid map set forth in a figure selected from the group consisting of FIGS. 1-10.
A method for expressing a target polypeptide encoded by a gene which is operably associated with a promoter, in a host cell, comprising introducing a βGl-IgG intron polynucleotide comprising a beta-globin splice donor site and an immunoglobulin gamma splice acceptor wherein said sites are separated by about 125 nucleotides, between the promoter and the polynucleotide encoding the target polypeptide, into the host cell, and culturing the host cell under conditions whereby the target polypeptide is expressed.
Dependencies are inferred from the claim text.