TCR Complex Immunotherapeutics
AssigneeEmergent Product Development Seattle, LLC
InventorsValerie Odegard, Catherine J. McMahan, Peter Robert Baum, Peter Armstrong Thompson, Philip Tan, John W. Blankenship, Sateesh Kumar Natarajan
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Abstract
Single chain fusion proteins that specifically bind to a TCR complex or a component thereof, such as TCRα, TCRβ, or CD3ε, along with compositions and methods of use thereof are provided.
Claims33
A single chain fusion protein, comprising from amino-terminus to carboxy-terminus:
- (a) a binding domain that specifically binds to a TCR complex or a component thereof,
- (b) a linker polypeptide, and
- (c) an immunoglobulin CH3 region polypeptide,
- wherein the fusion protein does not induce or induces a minimally detectable cytokine release.
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A method for detecting cytokine release induced by a protein that comprises a binding domain that specifically binds to a TCR complex or a component thereof, comprising:
- (a) providing mitogen-primed T cells,
- (b) treating the primed T cells of step (a) with the protein that comprises a binding domain that specifically binding to a TCR complex or a component thereof, and
- (c) detecting release of a cytokine from the primed T cells treated in step (b).
A method for detecting T cell activation induced by a protein that comprises a binding domain that specifically binds to a TCR complex or a component thereof, comprising:
- (a) providing mitogen-primed T cells,
- (b) treating the primed T cells of step (a) with the protein that comprises a binding domain that specifically binding to a TCR complex or a component thereof, and
- (c) detecting activation of the primed T cells treated in step (b).
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A single chain fusion protein, comprising from amino-terminus to carboxy-terminus:
- (a) a single chain Fv (scFv) binding domain polypeptide comprising the amino acid sequence of SEQ ID NO:263;
- (b) an immunoglobulin hinge region polypeptide comprising amino acids 3-17 of SEQ ID NO:10;
- and
- (c) an immunoglobulin CH3 region polypeptide.
Dependencies are inferred from the claim text.