Methods for production and uses of multipotent ,pluripotent, differentiated and disease-resistant cell populations

A method of cellular reprogramming for the interconversion of a differentiated or somatic cell of a first cellular phenotype to a second, multipotent, pluripotent, self-renewing or differentiating phenotype, the method comprising:

• growing said cell in a growth medium containing one or more cytokines selected from EGF, IL-7, oncostatin, CNTF, soluble gp130, bFGF, steel factor, LIF, cardiotrophin, OSM, IL6, hyper IL6, a cytokine having LIF activity, and other growth enhancing cytokines, effective that the selected cells grow at a first growth rate;
• contacting said cell in a contacting step comprising contacting said cell with a polypeptide, or with ribonucleic acids or deoxyribonucleic acids encoding one or more polypeptides, wherein said one or more of said polypeptides promotes desirable differentiation;
• genetically-modifying said cell with a nucleotide sequence, polypeptide, or nucleic acid encoding a polypeptide;
• incubating said cell in a differentiation medium comprising at least one differentiation agent conducive to promote differentiation of said cell into a desired differentiating cell;
• assessing said cell according to morphology, expression of cellular markers, transgenic markers, antibiotic markers, or fluorescent markers;
• and
• transplanting said cell to an individual or to a patient in need thereof.

A method of cellular reprogramming for the interconversion of a differentiated or somatic cell of a first cellular phenotype to a second, multipotent, pluripotent, self-renewing or differentiating phenotype, the method comprising:

• contacting a nucleated cell in a first contacting step with a first polypeptide or vector encoding a first polypeptide in a first contacting step, said first contacting step comprising contacting said cell with said first polypeptide, or with one or more ribonucleic acids or deoxyribonucleic acids encoding said first polypeptide, wherein said first polypeptide is Oct3/4, Sox2, LIN28 or Nanog;
• further contacting said cell in a second contacting step with one or more second polypeptides, or with ribonucleic acids or deoxyribonucleic acids encoding one or more second polypeptides, wherein said one or more second polypeptide is selected from Numb, Notch, FGF4, HOXB4, LIF, LIFR, CNTF, cardiotrophin, OSM, OSM-R, IL6, IL6R, hyper IL6, IL-11, gp130, stat3, c-myc, and a polypeptide with LIF activity;
• growing said cell in a growth medium containing one or more cytokines selected from EGF, IL-7, oncostatin, CNTF, soluble gp130, bFGF, steel factor, LIF, cardiotrophin, OSM, IL6, hyper IL6, a cytokine having LIF activity, and other growth enhancing cytokines, effective that said cell grows at a first growth rate;
• contacting said cell in a third contacting step comprising contacting said cell with a third polypeptide, or with ribonucleic acids or deoxyribonucleic acids encoding one or more third polypeptides, wherein said one or more third polypeptide promotes desirable differentiation;
• genetically-modifying said cell with a nucleotide sequence, polypeptide, or nucleic acid encoding a polypeptide;
• incubating said cell in a differentiation medium comprising at least one differentiation agent conducive to promote differentiation of said cell into a desired differentiating cell;
• assessing said cell according to morphology, expression of cellular markers, transgenic markers, antibiotic markers, or fluorescent markers;
• and
• transplanting said cell to an individual or to a patient in need thereof.