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US 2011/0216622 A1Published application
Liposomal apparatus and manufacturing method
AssigneeProtiva Biotherapeutics, Inc.
InventorsIan MacLachlan, Lloyd Jeffs, Lorne R. Palmer, Cory Giesbrecht, Noelle Giesbrecht
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Abstract
The present invention provides apparatus and processes for producing liposomes. By providing a buffer solution in a first reservoir, and a lipid solution in a second reservoir, continuously diluting the lipid solution with the buffer solution in a mixing chamber produces a liposome. The lipid solution preferably comprises an organic solvent, such as a lower alkanol.
Claims13
1-56. (canceled)
An apparatus for producing a lipid vesicle encapsulating a nucleic acid within the lipid vesicle, said apparatus comprising:
- a first reservoir for holding an aqueous solution including a nucleic acid;
- a second reservoir for holding an organic lipid solution, wherein the lipids present in said organic lipid solution are solubilized in a lower alkanol at a concentration of about 75% v/v to 100% v/v;
- and
- a pump mechanism configured to pump said aqueous solution and said organic lipid solution into a mixing environment at about equal flow rates;
- wherein said organic lipid solution mixes with said aqueous solution in the mixing environment to instantaneously produce a lipid vesicle encapsulating the nucleic acid within the lipid vesicle by diluting said lower alkanol to a concentration of between 45% v/v to about 60% v/v;
- and
- wherein the mixing environment includes a T-connector, wherein said aqueous solution and said organic lipid solution are introduced into the T-connector as opposing flows at about 180° relative to each other and mixed within the T-connector.
4 more elements
Dependencies are inferred from the claim text.