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AssigneeCarl Zeiss Micro Imaging GmbH
InventorsMichael KEMPE, Gerhard KRAMPERT, Matthias WALD, Ralf WOLLESCHENSKY
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A method and arrangement for collimated microscopic imaging, including a first illumination of a sample in at least one region for exciting fluorescence, and a spatially resolving detection of the sample light by detector elements, the detection being associated with the region, wherein by means of a second illumination a sub-division of the region into separate fluorescent partial regions occurs, which are associated with the detector elements. The separation of the partial regions is carried out by the spatial separation of the fluorescent regions by means of intermediate regions having reduced fluorescence or no fluorescence, and/or by means of different spectral properties of the fluorescence from the partial regions.
A method for collimated microscopic imaging with a first illumination of a sample in at least one region for exciting fluorescence and a spatially resolving detection of the sample light by detector elements which is associated with the region, comprising separating said region by a second illumination into separate fluorescing partial regions which are associated with said detector elements, wherein said separating of said region into partial regions comprises spatially dividing said fluorescing regions by means of intermediate regions with reduced fluorescence or no fluorescence and/or by different spectral properties of the fluorescence from said partial regions.
Method for operating a fluorescence microscope, wherein a structured illumination of the sample is carried out, comprising a first detecting of luminescing sample points, spatially offsetting of the illumination distribution on the sample, a second detecting of luminescing sample points, and determining and storing the position of the detected sample points on a compiled sample image from the values of the spatial offset.
An arrangement for collimated microscopic imaging comprising at least two light sources for respectively at least a first illumination and second illumination of a sample, means for generating a patterned light distribution on said sample, at least one detector which is spatially resolving in at least one direction and has a plurality of detector elements, wherein fluorescence is excited by a patterned or spatially structured light distribution in at least N partial regions of said sample which are separated from one another, and wherein said partial regions are imaged on the detector in such a way that a definitive association of partial regions and detector elements is carried out.
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