Subcellular analysis by laser ablation electrospray ionization mass spectrometry
AssigneeThe George Washington University
InventorsAkos Vertes, Jessica A. Stolee, Bindesh Shrestha
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Abstract
In various embodiments, a method of laser ablation electrospray ionization mass spectrometry (LAESI-MS) may generally comprise micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein, ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume, intercepting the ablation plume by an electrospray plume to form ions, and detecting the ions by mass spectrometry.
Claims20
A method of laser ablation electrospray ionization mass spectrometry, the method comprising:
- micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein;
- ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume;
- intercepting the ablation plume by an electrospray plume to form ions;
- and
- detecting the ions by mass spectrometry.
A method of in situ analysis of a cell having a native water concentration, the method comprising:
- micro-dissecting the cell to expose at least one subcellular component therein comprising at least one chemical species of interest;
- subjecting the at least one subcellular component to laser ablation electrospray ionization mass spectrometry;
- and
- determining, by laser ablation electrospray ionization mass spectrometry, a relative intensity of a signal of the chemical species.
Dependencies are inferred from the claim text.