Microfluidic device and method of determining nucleotide sequence of target nucleic acid using the same

A microfluidic device comprising:

• at least one first channel;
• and at least one second channel or chamber connected to the first channel via a nanopore in a fluid communication manner, wherein a first electrode and a second electrode are disposed in the first channel for applying a voltage in the lengthwise direction of the first channel, a first detector that detects a material in the first channel is disposed over the first channel, a third electrode is disposed in the second channel or chamber to be paired with the first or second electrode for applying a voltage between the first and third electrodes or between the second and third electrodes, and a second detector that detects a material passing through the nanopore is disposed in the nanopore.