Sugar and lipid metabolism regulators in plants ii
AssigneeBASF Plant Science GmbH
InventorsVolker Mittendorf, Heiko Haertel, Petra Cirpus
Life of this patent
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Abstract
Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana are provided. The nucleic acids and proteins are used in methods of producing transgenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches or seed storage proteins.
Claims23
A method of producing a transgenic plant having a modified level of a seed storage compound comprising, transforming a plant cell with an expression vector comprising a lipid metabolism protein (LMP) nucleic acid and generating from the plant cell the transgenic plant, wherein the nucleic acid encodes a polypeptide that functions as a modulator of a seed storage compound in the plant, and wherein the LMP nucleic acid comprises
- a) the polynucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:43, or SEQ ID NO:45;
- b) a polynucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:44, or SEQ ID NO:46;
- c) a polynucleotide sequence of least 60 consecutive nucleotides of the polynucleotide sequence of a) or b);
- d) a polynucleotide sequence having at least 70% sequence identity with the polynucleotide sequence of a) or b) above;
- e) a polynucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 70% sequence identity with the sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:44, or SEQ ID NO:46;
- f) a polynucleotide sequence that hybridizes under stringent conditions to the complement of the polynucleotide sequence of a) or b) above;
- or
- g) a polynucleotide complementary to the polynucleotide sequence of a) or b) above.
A method of modulating a level of a seed storage compound in a plant comprising, modifying the expression of a lipid metabolism protein (LMP) nucleic acid in a plant, wherein the LMP nucleic acid comprises
- a) a polynucleotide sequence selected from the group consisting of:
- SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:43, and SEQ ID NO:45;
- b) a polynucleotide sequence encoding a polypeptide selected from the group consisting of:
- SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:44, and SEQ ID NO:46;
- c) a polynucleotide sequence of least 60 consecutive nucleotides of the polynucleotide sequence of a) or b);
- d) a polynucleotide sequence having at least 70% sequence identity with the polynucleotide sequence of a) or b) above;
- e) a polynucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 70% sequence identity with the sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:44, or SEQ ID NO:46;
- f) a polynucleotide sequence that hybridizes under stringent conditions to the complement of the polynucleotide sequence of a) or b) above;
- or
- g) a polynucleotide complementary to the polynucleotide sequence of a) or b) above.
An isolated lipid metabolism protein (LMP) nucleic acid comprising a polynucleotide sequence encoding a polypeptide that functions as a modulator of a seed storage compound in a plant, wherein the polynucleotide comprises
- a) the polynucleotide sequence of SEQ ID NO:5, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:33, or SEQ ID NO:37;
- b) a polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:6, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:18, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:34, or SEQ ID NO:38;
- c) a polynucleotide sequence of least 60 consecutive nucleotides of the polynucleotide sequence of a) or b);
- d) a polynucleotide sequence having at least 70% sequence identity with the polynucleotide sequence of a) or b) above;
- e) a polynucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 70% sequence identity with the sequence of SEQ ID NO:6, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:18, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:34, or SEQ ID NO:38;
- e) a polynucleotide sequence that hybridizes under stringent conditions to the complement of the polynucleotide sequence of a) or b) above;
- or
- f) a polynucleotide complementary to the polynucleotide sequence of a) or b) above.
Dependencies are inferred from the claim text.