Development of herbicide-resistant grass species

A method of identifying a herbicide-resistant plant from the group Panicodae, comprising:

• providing a callus of undifferentiated cells of a plant from the group Panicodae;
• contacting the callus with an acetyl coenzyme A carboxylase (ACCase) inhibitor in an amount sufficient to retard growth or kill the callus;
• selecting at least one resistant cell based upon a differential effect of the ACCase inhibitor;
• and
• regenerating a viable whole plant of the variety from the at least one resistant cell,
• wherein the regenerated plant is resistant to an acetyl coenzyme A carboxylase (ACCase) inhibitor.

A method of marker-assisted breeding, comprising the steps of:

• identifying a feature of interest for breeding and selection, wherein the feature is in linkage with an ACCase gene;
• providing a first plant carrying an ACCase sequence variant capable of conferring upon the plant resistance to an ACCase-inhibitor herbicide, wherein the plant further comprises the feature of interest;
• breeding the first plant with a second plant;
• identifying progeny of the breeding step as having the ACCase sequence variant;
• and
• selecting progeny likely to have the feature of interest based upon the identifying step, wherein the ACCase sequence variant comprises a variation at least one of position:
• 1756, 1781, 1999, 2027, 2041, 2078, 2099 and 2096.