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US 2011/0213288 A1Published application
Device And Method For Transfecting Cells For Therapeutic Uses
AssigneesBoard of Regents, The University of Texas System, William Marsh Rice University
InventorsYoonsu Choi, Lawrence J.N. Cooper, Dean A. Lee, Sibani Lisa Biswal, Robert Raphael, Thomas C. Killian
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Abstract
This invention generally relates to devices and methods for ex vivo or in vivo transfection of living cells using electroporation, in particular high throughput microfluidic electroporation, and to therapeutic uses of the transfected cells.
Claims37
A device, comprising:
- a base unit having a top surface and a bottom surface essentially parallel to and opposite the top surface;
- a first reaction tier comprising a plurality of microfluidic chambers impressed into the base unit, each chamber being defined by one or more side walls and a floor and having dimensions that permit the chamber to hold one intact eukaryotic cell;
- wherein:
- each chamber has a port extending from approximately the center of the floor of the chamber to the bottom surface of the base unit, where the port is capable of fluidic connection with an external source;
- and
- each chamber has one or more additional ports extending from the floor of the chamber to the bottom surface of the base unit, where each additional port is individually capable of fluidic connection with an external source;
- each chamber has a positive electrode and negative electrode operatively coupled to its wall(s) wherein the electrodes are disposed substantially opposite one another.
4 more elements
A device comprising:
- an orifice plate having an inlet surface, an outlet surface and an outer edge having a thickness;
- one or more through-holes extending through the orifice plate from the inlet surface to the outlet surface, the surface between the inlet and outlet surfaces comprising a wall surface;
- wherein
- each through-hole is sized to permit a single eukaryotic cell at a time pass through;
- each through-hole has a positive electrode operatively coupled to its wall surface substantially opposite a negative electrode likewise operatively coupled to its wall surface;
- a positive electrode connection and an negative electrode connection operatively coupled to the outer edge of the orifice plate, the positive electrode connection being operatively coupled to each positive electrode in each through-hole and the negative electrode connection being operatively coupled to each negative electrode in each through-hole;
- an inlet exterior source connector operatively coupled to the inlet surface of the orifice plate;
- and
- an outlet connector operatively coupled to the outlet surface of the orifice plate.
6 more elements
Dependencies are inferred from the claim text.