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US 2011/0213126 A1Published application
Two-stage ultrafiltration/diafiltration
AssigneeTalecris Biotherapeutics, Inc.
InventorsMartin Gonzalez, Woody D. Wood, Fred H. Earp
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Abstract
The present invention provides a method for concentrating a protein, in particular a method for concentrating a plasma product, in particular IgG, using glycine in a (two-stage ultrafiltration/diafiltration approach.
Claims15
A method for concentrating a plasma product of a solution comprising the protein, the method comprising:
- a) ultrafiltering the solution using a first membrane to form a first retentate solution comprising the protein at a first concentration, wherein the first membrane has a molecular weight cutoff sufficient to retain at least a portion of the protein present in the solution;
- b) diafiltering the first retentate solution with an aqueous solution using the first membrane to form a second retentate solution comprising the protein at about the first concentration;
- c) formulating the second retentate comprising the diafiltered protein with glycine and adjusting the pH;
- and
- d) ultrafiltering the second retentate solution using a second membrane to form a final retentate solution comprising the protein at a second concentration, wherein the second membrane has a molecular weight cutoff of about twice the molecular weight cutoff of the first membrane, wherein the second concentration is greater than the first concentration.
2 more elements
A method for concentrating a protein of a solution comprising the protein, the method comprising:
- a) ultrafiltering the solution using a first membrane to form a first retentate solution comprising the protein at a first concentration of about 5%, wherein the first membrane has a molecular weight cutoff sufficient to retain at least about 90% of the protein;
- b) diafiltering the first retentate solution using the first membrane with water to form a second retentate solution comprising the protein at about the first concentration;
- c) formulating the about 5% diafiltered protein of step b) to about 0.16 to about 0.30 M glycine, wherein the formulating further comprises adjusting pH to about 4.3;
- and
- d) ultrafiltering the second retentate solution using a second membrane to form a final retentate solution comprising the protein at a second concentration of about 19 to about 21%, wherein the second membrane has a molecular weight cutoff of about twice the molecular weight cutoff of the first membrane, wherein the second concentration is greater than the first concentration.
2 more elements
Dependencies are inferred from the claim text.