Back to results
US 2011/0213124 A1Published application
Methods and systems for synthesis of a d-aminoluciferin precursor and related compounds
OwnerAmy L. GRYSHUK, Julie Perkinsinventors
Life of this patent
Estimating the term…
Abstract
Methods and systems to generate 6-amino-6-deoxy-D-luciferin precursor, 2-cyano-6-aminobenzothiazole and related compounds and derivatives
Claims12
A method to provide a peptide conjugated to 6-amino-6-deoxy-D-luciferin, the method comprising
- conjugating an amino acid with a 2-cyano-6-aminobenzothiazole to provide a 2-cyano-6-amino-aminoacid-benzothiazole, the conjugating performed to allow formation of a bond between the amino group of 6-amino-6-deoxy-D-luciferin and a carboxylic group of the amino acid;
- conjugating the peptide with the 2-cyano-6-amino-aminoacid-benzothiazole to provide a 2-cyano-6-amino-peptide-benzothiazole;
- and
- reacting the 2-cyano-6-amino-peptide-benzothiazole with a D-cysteine to provide a 6-amino-peptide-6-deoxy-D-luciferin,
- wherein the bond is a peptide bond, carbamate bond or a urea/thiourea bond.
2 more elements
An intermediate in the synthesis of an amino acid labeled with 6-amino-6-deoxy-D-luciferin, the intermediate being a 2-cyano-6-aminobenzothiazole of formula (III)
- wherein R1 is a single amino acid and R2 is a peptide attached to the remainder of the compound of formula (IV) through a peptide bond a carbamate bond or a urea/thiourea bond.
Dependencies are inferred from the claim text.