Compositions and Methods for Treating Cancer, Inhibiting Proliferation, and Inducing Cell Death
AssigneeUniversity of Louisville Research Foundation, Inc.
InventorsDonald M. Miller, Shelia Diann Thomas, Kara Joyce Sedoris
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Abstract
Quadruplex-forming guanine-rich nucleic acid sequences are useful in compositions and methods for inhibiting cellular growth and proliferation and inducing cell death. Compositions for treating a patient are provided, including (i) a safe and effective amount of a sequence having at least 80% nucleic acid identity with a guanine-rich promoter gene oligonucleotide (GPGO), and (ii) a carrier, wherein the oligonucleotide forms at least one quadruplex.
Claims19
A composition for treating a patient, comprising:
- (i) a safe and effective amount of at least one oligonucleotide comprising a sequence having at least 80% nucleic acid sequence identity with a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:10, and combinations thereof, and
- (ii) a carrier,
- wherein the oligonucleotide forms at least one quadruplex.
A composition for treating a patient, comprising:
- (i) a safe and effective amount of at least two oligonucleotides, each oligonucleotide comprising a sequence having at least 80% nucleic acid sequence identity with a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, and SEQ ID NO:10, and
- (ii) a carrier,
- wherein each of said oligonucleotides forms at least one quadruplex.
A composition for treating a patient, comprising:
- (i) a safe and effective amount of at least three oligonucleotides, each oligonucleotide comprising a sequence having at least 80% nucleic acid sequence identity with a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, and SEQ ID NO:10, and
- (ii) a carrier,
- wherein each of said oligonucleotides forms at least one quadruplex.
A method of treating a patient having a tumor, the method comprising:
- (a) performing a biopsy of the patient's tumor;
- (b) determining a gene expression profile of the tumor;
- (c) identifying one or more genes that are overexpressed in the tumor, based on the gene expression profile of step (b);
- (d) selecting one or more GPGO sequences corresponding to the overexpressed genes identified in step (c);
- (e) administering to the patient a composition comprising a safe and effective amount of the one or more GPGO sequences of step (d).
Dependencies are inferred from the claim text.