Oligonucleotidic sequences able to silence the expression of the cyclin d1-trop2 chimera and uses thereof in medical field
AssigneeSaverio ALBERTI
InventorsSaverio Alberti, Emanuela Guerra
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Abstract
The invention concerns RNA oligonucleotide sequences or sequences that are transcribed into RNA or analogous molecules able to silence the expression of the CYCLIN D1/TROP2 chimeric mRNA and their use in the treatment and the prevention of tumors.
Claims12
Oligonucleotide sequence (siRNA) able to silence the expression of the CYCLIN D1/TROP2 chimeric mRNA, said siRNA being characterized by the fact that is a ribonucleotide sequence complementary to the CYCLIN D1/TROP2 chimeric mRNA comprising or having the following CYCLIN D1/TROP2 junction sequence 5′GAGAGAGAGAGAAAGGAGCCC 3′ (SEQ ID NO:21)
Kit for the in-vitro detection and quantification of the CYCLIN D1/TROP2 chimera in a biological sample comprising or consisting in the following PCR primers having the following sequence:
- (SEQ ID NO:
- 4)
- (forward)
- 5′ CTGGCCGCAATGACCCCGCA 3′
- (SEQ ID NO:
- 9)
- (reverse)
- 5′ GGCGGAGGAACGCGGACCGG 3′
- (SEQ ID NO:
- 5)
- (forward)
- 5′ GCGGGATCCAAGGGAAAGCTTCATTCT 3′
- (SEQ ID NO:
- 10)
- (reverse)
- 5′ GAGGCGCGGGGACTCGTCGG 3′
- (SEQ ID NO:
- 6)
- (forward)
- 5′ GCGGGATCCCCTTGTTGTTGGTTGTTT 3′
- preferably,
- (SEQ ID NO:
- 5)
- (forward)
- 5′ GCGGGATCCAAGGGAAAGCTTCATTCT 3′
- (SEQ ID NO:
- 10)
- (reverse)
- 5′ GAGGCGCGGGGACTCGTCGG 3′
Method for the in-vitro detection and quantification of the CYCLIN D1/TROP2 chimera in a biological sample using the following primers for real-time PCR:
- (SEQ ID NO:
- 4)
- (forward)
- 5′ CTGGCCGCAATGACCCCGCA 3′
- (SEQ ID NO:
- 9)
- (reverse)
- 5′ GGCGGAGGAACGCGGACCGG 3′
- (SEQ ID NO:
- 5)
- (forward)
- 5′ GCGGGATCCAAGGGAAAGCTTCATTCT 3′
- (SEQ ID NO:
- 10)
- (reverse)
- 5′ GAGGCGCGGGGACTCGTCGG 3′
- (SEQ ID NO:
- 6)
- (forward)
- 5′ GCGGGATCCCCTTGTTGTTGGTTGTTT 3′
- preferably,
- (SEQ ID NO:
- 5)
- (forward)
- 5′ GCGGGATCCAAGGGAAAGCTTCATTCT 3′
- (SEQ ID NO:
- 10)
- (reverse)
- 5′ GAGGCGCGGGGACTCGTCGG 3′
Dependencies are inferred from the claim text.