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US 2011/0212876 A1Published application
Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent
AssigneeThe Procter & Gamble Company
InventorsMichelle MEEK, Philip Frank Souter, Lindsay Suzanne Bewick, Allan Svendsen, Annette Helle Johansen, Mads Eskelund Bjoernvad, Frank Winther Rasmussen, Michael Skjoet, Signe Eskildsen Larsen, Jens Oebro, Svend Kaasgaard, Lars Beier
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Abstract
The present invention relates to cleaning compositions comprising variants of an alpha-amylase having improved stability to chelating agents relative to its parent enzyme and cleaning processes comprising such compositions.
Claims15
A cleaning composition comprising:
- (a) a variant of a parent alpha-amylase wherein the variant comprises a substitution at one or more positions selected from the group consisting of 195, 193, 197, 198, 200, 203, 206, 210, 212, 213 and 243, using the numbering according to SEQ ID NO:
- 6;
- and
- (b) a cleaning adjunct, preferably in an amount from 0.01 to 99.9 wt %;
- and optionally
- (c) at least one chelating agent wherein said chelating agent at a concentration below 10 mM is capable of reducing the concentration of free calcium ions from 2.0 mM to 0.10 mM when measured at 21° C. and pH 8.0.
3 more elements
A cleaning composition comprising:
- (a) a variant of a parent alpha-amylase wherein the variant comprises a substitution at one or more positions selected from the group consisting of 195, 193, 197, 198, 200, 203, 206, 210, 212, 213 and 243, using the numbering according to SEQ ID NO:
- 6;
- and
- (b) a cleaning adjunct, preferably in an amount from 0.01 to 99.9 wt %;
- and optionally
- (c) at least one chelating agent wherein said chelating agent is capable of reducing the free calcium ion concentration from 2.0 mM to 0.10 mM at a chelating agent concentration below 0.9 times the concentration of citrate capable of reducing the free calcium ions from 2.0 mM to 0.10 mM, when measured at 21° C. and pH 8.0.
3 more elements
Dependencies are inferred from the claim text.