High-throughput platform for in-vivo sub-cellular screens on vertebrate larvae
AssigneeMassachusetts Institute of Technology
InventorsMehmet F. Yanik, Steven C. Wasserman, Carlos Pardo, Cody L. Gilleland, Tsung-Yao Chang
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Abstract
High throughput system for in vivo screens on vertebrate larvae. The system includes a source of vertebrate larvae in a liquid medium and loading tube means for aspirating a larva. A detector assembly is provided to differentiate passage of a larva from bubbles and/or debris. An imaging means is provided for both confocal imaging and wide-field fluorescence imaging of the larva. A laser is provided for optical manipulation of the larva.
Claims43
High throughput system for in vivo screens comprising:
- a source of specimen organisms in a liquid medium;
- an automatic means to load the specimen organisms from the source into a tube;
- an automatic means to position the specimen organism within the field-of-view of an imaging apparatus;
- and
- a means to rotate the specimen organism.
Method for high-throughput in vivo screens comprising:
- providing a source of specimen organisms in a liquid medium;
- automatically loading the specimen organisms from the source;
- automatically positioning the specimen organisms within the field-of-view of an imaging apparatus;
- and
- rotating the specimen organisms.
An automated system for in vivo screens on vertebrate larvae comprising:
- a source of vertebrate larvae in a liquid medium;
- means to aspirate a larva into a tube;
- a detection means to differentiate passage of a larva from bubbles and/or debris;
- a means to rotate the organism;
- and
- a means for imaging the organism.
Dependencies are inferred from the claim text.