Structure based and combinatorially selected oligonucleoside phosphorothioate and phosphorodithioate aptamer targeting ap-1 transcription factors
AssigneeBoard of Regents, The University of Texas System
InventorsDavid G. Gorenstein, Bruce A. Luxon, James Leary
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Abstract
The present invention includes composition and methods for making and using a combinatorial library to identify modified thioaptamers that bind to, and affect the immune response of a host animal, transcription factors such as IL-6, NF-κB, AP-1 and the like. Composition and methods are also provided for the treatment of viral infections, as well as, vaccines and vaccine adjuvants are provided that modify host immune responses.
Claims45
A bead-based method for identifying both thioaptamer sequences and binding proteins comprising the steps of:
- incubating a thioaptamer library with a sample suspected of comprising one or more proteins;
- selecting the one or more thioaptamers of the library to which protein has bound;
- identifying the proteins by mass spectrometry;
- and
- identifying the thioaptamer by PCR, cloning and sequencing.
A system for identifying proteins that interact with a specific thioaptamer comprising:
- a thioaptamer library for binding one or more proteins;
- a bead separator wherein the one or more thioaptamer beads of the library to which protein has bound are separated;
- a mass spectrometer to identify the proteins that have bound to the one or thioaptamer beads.
A bead-based method for identifying one or more proteins that bind to one or more thioaptamers comprising the steps of:
- incubating a thioaptamer library with a sample suspected of comprising one or more proteins;
- and
- identifying the proteins by mass spectrometry.
A method for identifying one or more targets that bind to one or more thioaptamers comprising the steps of:
- incubating a thioaptamer library with a sample suspected of comprising one or more targets;
- and
- identifying the target by mass spectrometry.
Dependencies are inferred from the claim text.