Method and Reagent Kit for Immunological Measurement
AssigneeAlfresa Pharma Corporation
InventorMutsumi Tanaka
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Abstract
A method for measuring an analyte in a sample, includes the steps of (a) mixing a sample containing the analyte, a complex comprising a plurality of molecules of a substance that specifically binds to the analyte, and microparticles each having the analyte or an analog of the analyte bound to an insoluble support; and (b) measuring an agglutination reaction of the microparticles in a mixture obtained in the step (a). In the method, for example, an antibody is used in the form of a complex of a plurality of molecules of the antibody, rather than in the form of a single molecule of the antibody. Therefore, the sensitivity to the measurement is increased, and the measurement in a lower concentration range becomes possible. A reagent kit for measuring is also disclosed.
Claims13
A method for measuring an analyte in a sample, the method comprising:
- (a) mixing:
- a sample containing the analyte,
- a complex comprising a plurality of molecules of a substance that specifically binds to the analyte, and
- microparticles each having the analyte or an analog of the analyte bound to an insoluble support;
- and
- (b) measuring an agglutination reaction of the microparticles in a mixture obtained in the step (a).
A reagent kit for measurement comprising:
- a first reagent, which comprises a complex comprising a plurality of molecules of a substance that specifically binds to an analyte;
- and
- a second reagent, which comprises microparticles each having an analyte or an analog of the analyte bound to an insoluble support.
Dependencies are inferred from the claim text.