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US 2011/0212543 A1Published application
Method for preparing agglutinatable platelet fragments and the use thereof
AssigneeSiemens Healthcare Diagnotics Products GMBH
InventorJürgen PATZKE
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Abstract
The present invention relates to a method for preparing agglutinatable platelet fragments, in which native platelets are treated with ultrasound and a fixative. The platelet fragments are suitable for use in diagnostic assay methods which include an agglutination reaction, such as, for example, in a method for determining WWF activity.
Claims21
1-6. (canceled)
A method for determining the von Willebrand factor IMF) activity in a sample, which comprises:
- (a) mixing the sample with agglutinatable platelet fragments which have been prepared by a method comprising:
- (i) treating a suspension of native platelets with ultrasound to fragment the platelets, and then,
- (ii) adding a fixative to the suspension of platelet fragments from step (i), and
- (b) determining the agglutination reaction of the platelet fragments in the reaction mixture.
1 more element
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